Abbreviations

cDNA

complementary DNA

DEAE

diethylaminoethyl

DHFR

dihydrofolate reductase

DPM

disintegrations per minute

DTT

dithiothreitol

DxD

aspartate-any amino acid-aspartate

EDS VI

Ehlers-Danlos syndrome type VI

ER

endoplasmic reticulum

Gal

galactose

GFP

green fluorescent protein

GGT

galactosylhydroxylysyl glucosyltransferase

Glc

glucose

GT

hydroxylysyl galactosyltransferase

Hyp

hydroxylprolyl residue

Hyl

hydroxylysyl residue

IPTG

isopropyl-β -D-thiogalactopyranoside

kDa

kilodalton(s)

KDEL

lysine-aspartate-glutamate-leucine

LH

lysyl hydroxylase

mRNA

messenger RNA

NTA

nitrilotriacetic acid

PAGE

polyacrylamide gel electrophoresis

PBS

phosphate-buffered saline

PCR

polymerase chain reaction

PH-4

prolyl 4-hydroxylase

PLOD

gene name for human lysyl hydroxylase

Plod

gene name for mouse lysyl hydroxylase

SDS

sodium dodecyl sulfate

UDP

uridine diphosphate

X

any amino acid

Y

any amino acid