| Δ3-Δ2-Enoyl-CoA isomerase from the yeast Saccharomyces cerevisiae: Molecular and structural characterization | ||
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The elution volume of Eci1p from the Superdex 200 HR10/30 gel filtration column was compared to that of dienoyl-CoA isomerase, a hexameric protein of 170 000 Da. Gel filtration was also used to find out whether the purified E158A mutant Eci1p has the same native molecular mass as the wild-type enzyme. The native size of Eci1p was also analyzed using the DynaPro dynamic light scattering device (Protein Solutions).
The native size of Eci1p at acidic and neutral pH was analyzed by sedimentation velocity experiments using an analytical ultracentrifuge (Beckman XL-A) by Dr. Arie Geerlof at the EMBL, Heidelberg, Germany.