| Δ3-Δ2-Enoyl-CoA isomerase from the yeast Saccharomyces cerevisiae: Molecular and structural characterization | ||
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The over-expression of full-length Eci1p using the pET expression system (Studier 1991) was performed according to the manufacturer’s instructions. The induction of protein production was carried out at 30°C. The cells were separated, suspended in lysis buffer (20 mM potassium phosphate, pH 7.6, 100 mM KCl) and stored at -70°C.