Abbreviations

ADME

Adsorption, Distribution, Metabolism, Excretion

cDNA

complementary deoxyribonucleic acid

Clint

intrinsic clearance

CYP

cytochrome P450

DME

drug-metabolising enzyme

ER

endoplasmic reticulum

FMO3

flavin-containing monooxygenase type 3

HPLC

high-performance liquid chromatography

HTS

high-throughput screening

[I];

concentration of inhibitor in enzymatic reaction

IC50

inhibitor concentration decreasing the enzymatic activity by 50% compared to the control reaction

Ki

inhibition constant

Km

Michaelis-Menten constant for a substrate

mRNA

messenger ribonucleic acid

MS

mass spectrometry

NADPH

nicotine amide diphosphate (reduced form)

NCE

new chemical entity, a drug under development

P450

cytochrome P450

PAH

polycyclic aromatic hydrocarbon

PM

poor metaboliser

r

correlation coefficient

[S];

concentration of substrate in enzymatic reaction

SER

smooth endoplasmic reticulum

t

in vitro half life of the compound under study

UDP

uridine diphosphate

V

velocity of metabolite formation in enzymatic reaction

Vmax

maximal reaction velocity