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Information from a series of urinary 3H measurements on each rat (in studies I, II and III) was summarized as the area under the curve as described by Altman (1990).
Statistical significances of the differences between the groups concerning all measured variables in studies I, II, IV and V were calculated using the analysis of variance, and further comparison was made using Fisher´s Protected Least Significant Difference (Armitage & Berry 1994).
In study III, statistical significances of the differences between the groups were calculated using unpaired t-test.
The statistical computer program used was StatView II for Macintosh (Abacus Concepts, Inc., Berkeley, CA).